Thingiverse
Autostainer for Biological Specimens 3D Printed USB Powered by sandrabaskharon
by Thingiverse
Last crawled date: 3 years, 1 month ago
3D printing is considered a transformational technology; however, its potential for configuring research laboratory equipment is nearly unprecedented. This project uses inexpensive and widely available electronic parts and a commercially available 3D printer to create a USB Powered Autostainer for Biological Specimens.
This platform uses two USB powered stepper motors (one turns the stain tanks into position under the slide and one dips the slide holding biological material into the stain tank), two stepper motor Driver boards, one Arduino UNO microcontroller, one LCD Shield and custom designed ABS plastic 3D printed structural components.
The microcontroller program polls buttons on the Shield to write a 5 step staining protocol. It is fully programmable from this LCD Shield user interface and does not need to be connected to a computer for program control. Each step is written into an element of an array for later program execution. The program first "Homes" the Dip and then the Tank stepper motor against limit switches. The program then executes each movement from the arrays which received the programming steps.
Future developments include inexpensive autostainer platforms for any high school program with access to a 3D printer as well as additional modules for the platform for example an auto-immunostainer or a Thermofoil heated Real Time PCR platform. Since the platform uses only USB power is conceivable that it could be powered by a small solar panel (for field research).
Automated Laberatory Equiptment
Automated systems are essential in both education and in research.
Essentially all laboratory equipment uses similar components (optics, fluidics, controlled movements, pneumatics and data management
Using 3D printed parts and frames allows you to rapidly prototype low cost automated laboratory equipment for Educational uses and for field research.
Being able to control the movements of automated systems in research or in education can be a tremendous advantage for creating automated systems
Using USB power allows lower power consumption (USB 4.5 V and 100 mA)
3D printed prototype platforms can be Scalable, Modular and Expandable
Stain for Testing the System
Diff-Quik is a commercial Romanowsky stain variant, commonly used in histological staining to rapidly stain and differentiate a variety of smears, commonly blood and non-gynecological smears, including those of fine needle aspirates. It is based on a modification of the Wright Giemsa stain pioneered by Bernard Witlin in 1970. It has advantages over the older Wright Giemsa staining technique, as it reduces the 4 minute process into a simplified 15 second operation, and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.
Diff-Quik is utilized on material which is air-dried prior to alcohol fixation .
Solutions required:
Fixative (Fast green in methanol) - pale green colour
Stain solution 1 (Eosin G in phosphate buffer) - red colour
Stain solution 2 (Thiazine dye in phosphate buffer) - blue colour
Method:
Allow smears to dry
Dip slide five times, for one second each, into Fixative.
Allow excess to drain after each dip.
Dip slide or tape-strip five times, for one second each, into Stain
Configuration:
A portable Up!3D printer was used to fabricate the 3D printed parts. The printer used the following parameters:
Z Resolution of 0.20mm, Base height 2 mm, Support Density 2 layers, 25% infill of parts interiors. The parts were printed using ABS plastic filament obtained form Octave.com. The parametric files for each object were saved in standard STL (stereolithography) file.
The Arduino UNO Microcontroller was selected because it permitted an adequate number of digital inputs and outputs to control this prototype while allowing expansion for future development of related systems.
The stepper motors needed to run with adequate force using USB power. The Steppers were wired as Bipolar motors and the Sparkfun Bipolar motor driver permitted control with the Arduino microcontroller.
The entire system was designed for low cost (<50$), reliability, low power consumption and portability.
Limitations:
Platform limited to staining a single slide staining at a time
This version is limited to 4 staining reservoirs or wells, maximum
There is no blotting pad or wipe between the staining dips
Changes in the microcontroller program require computer connection
The housing for the unit needs to protect the wiring
The electronics should be embedded in a cowling or cover
There is currently no way to change the distance that the slide travels
3D printed parts:
Slide guide -ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ guides glass slide
Limit switch holder (Tank) - Snap-Arm
Limit switch holder (Slide Dip) proximityÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ
Support wire guide - supports microscope slide
Arch - Rotates to Lower glass slide for staining
Stepper holder (Tank) rotates stain reservoirs
Tank rotation base, attached to Mentos holder
Reservoir holder for Mentos containers
FUture Platform Enhancements:
This flexible platform is:Automated, reliable, programmable, scalable, modular, expandable, inexpensive, with low power consumption, a small footprint and is assembled with 3D printed parts.
The next generation may include:
Auto Immunohistochemistry platform, by adding three syringe pumps.
In situ hybridization platform adding USB powered thermofoil heaters.
Linked modules to support more complex staining protocols
Labview control interface or Scratch S4A for more flexible control
This High school science project was overseen by:
Dr. R. Siderits at RWJUHH:
Awards
1) Office of Naval Research Naval Science Award Certificate and a medal
2) AMS Materials Education Foundation for Most Outstanding Exhibit in Materials Science
3) First Place in the Senior division in the field of Engineering Materials and Bioengineering
4) Technology Grant in the form of a microcontroller system called a Beagle board.
This platform uses two USB powered stepper motors (one turns the stain tanks into position under the slide and one dips the slide holding biological material into the stain tank), two stepper motor Driver boards, one Arduino UNO microcontroller, one LCD Shield and custom designed ABS plastic 3D printed structural components.
The microcontroller program polls buttons on the Shield to write a 5 step staining protocol. It is fully programmable from this LCD Shield user interface and does not need to be connected to a computer for program control. Each step is written into an element of an array for later program execution. The program first "Homes" the Dip and then the Tank stepper motor against limit switches. The program then executes each movement from the arrays which received the programming steps.
Future developments include inexpensive autostainer platforms for any high school program with access to a 3D printer as well as additional modules for the platform for example an auto-immunostainer or a Thermofoil heated Real Time PCR platform. Since the platform uses only USB power is conceivable that it could be powered by a small solar panel (for field research).
Automated Laberatory Equiptment
Automated systems are essential in both education and in research.
Essentially all laboratory equipment uses similar components (optics, fluidics, controlled movements, pneumatics and data management
Using 3D printed parts and frames allows you to rapidly prototype low cost automated laboratory equipment for Educational uses and for field research.
Being able to control the movements of automated systems in research or in education can be a tremendous advantage for creating automated systems
Using USB power allows lower power consumption (USB 4.5 V and 100 mA)
3D printed prototype platforms can be Scalable, Modular and Expandable
Stain for Testing the System
Diff-Quik is a commercial Romanowsky stain variant, commonly used in histological staining to rapidly stain and differentiate a variety of smears, commonly blood and non-gynecological smears, including those of fine needle aspirates. It is based on a modification of the Wright Giemsa stain pioneered by Bernard Witlin in 1970. It has advantages over the older Wright Giemsa staining technique, as it reduces the 4 minute process into a simplified 15 second operation, and allows for selective increased eosinophilic or basophilic staining depending upon the time the smear is left in the staining solutions.
Diff-Quik is utilized on material which is air-dried prior to alcohol fixation .
Solutions required:
Fixative (Fast green in methanol) - pale green colour
Stain solution 1 (Eosin G in phosphate buffer) - red colour
Stain solution 2 (Thiazine dye in phosphate buffer) - blue colour
Method:
Allow smears to dry
Dip slide five times, for one second each, into Fixative.
Allow excess to drain after each dip.
Dip slide or tape-strip five times, for one second each, into Stain
Configuration:
A portable Up!3D printer was used to fabricate the 3D printed parts. The printer used the following parameters:
Z Resolution of 0.20mm, Base height 2 mm, Support Density 2 layers, 25% infill of parts interiors. The parts were printed using ABS plastic filament obtained form Octave.com. The parametric files for each object were saved in standard STL (stereolithography) file.
The Arduino UNO Microcontroller was selected because it permitted an adequate number of digital inputs and outputs to control this prototype while allowing expansion for future development of related systems.
The stepper motors needed to run with adequate force using USB power. The Steppers were wired as Bipolar motors and the Sparkfun Bipolar motor driver permitted control with the Arduino microcontroller.
The entire system was designed for low cost (<50$), reliability, low power consumption and portability.
Limitations:
Platform limited to staining a single slide staining at a time
This version is limited to 4 staining reservoirs or wells, maximum
There is no blotting pad or wipe between the staining dips
Changes in the microcontroller program require computer connection
The housing for the unit needs to protect the wiring
The electronics should be embedded in a cowling or cover
There is currently no way to change the distance that the slide travels
3D printed parts:
Slide guide -ÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ guides glass slide
Limit switch holder (Tank) - Snap-Arm
Limit switch holder (Slide Dip) proximityÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂÃÂ
Support wire guide - supports microscope slide
Arch - Rotates to Lower glass slide for staining
Stepper holder (Tank) rotates stain reservoirs
Tank rotation base, attached to Mentos holder
Reservoir holder for Mentos containers
FUture Platform Enhancements:
This flexible platform is:Automated, reliable, programmable, scalable, modular, expandable, inexpensive, with low power consumption, a small footprint and is assembled with 3D printed parts.
The next generation may include:
Auto Immunohistochemistry platform, by adding three syringe pumps.
In situ hybridization platform adding USB powered thermofoil heaters.
Linked modules to support more complex staining protocols
Labview control interface or Scratch S4A for more flexible control
This High school science project was overseen by:
Dr. R. Siderits at RWJUHH:
Awards
1) Office of Naval Research Naval Science Award Certificate and a medal
2) AMS Materials Education Foundation for Most Outstanding Exhibit in Materials Science
3) First Place in the Senior division in the field of Engineering Materials and Bioengineering
4) Technology Grant in the form of a microcontroller system called a Beagle board.
Similar models
thingiverse
free
Filament guide replacement for Eventorbot direct extruder by Sungod3k
...re. this one a problem in the first version (http://www.thingiverse.com/thing:172076) and should be substantially reduced by now.
thingiverse
free
Integrated Spool Holding System for the Felix Robotics Printer by Pacificimc
...ãâãâ¢ãâãâãâãâãâãâãâãâsolid and very little filament resistanceãâãâãâãâ¢ãâãâãâãâãâãâãâãâ
thingiverse
free
coremic by mic
...tepper con planetario dell'asse z per questo tipo di stampanti ( esistino altri tipi di stampanti con movimenti di z simili)
grabcad
free
linear slide stepper motor with l2 98 driver
... stepper motor with l2 98 driver
grabcad
a linear moving system using linear slide stepper motor with a l2 98 powered by battery
thingiverse
free
MobyStick - Whale USB and SD holder by TwistedDaylight
...wanting to make their own stuff.
06.02.14 updated the files, now the sd and usb slots have 0,4mm tolerance to allow proper fit.
blendswap
free
pearHI
... ãâ¬ãâµãâ°ãâ»ãâ¸ãâãâãâ¸ãâ¡ãâ½ãâ¾ãâ¹.latest change on 2013/04/19, 08:58:52ðºð¾ññðµðºñð¸ñð¾ð²ðºð° ñðµðºññð°
thingiverse
free
Shrek (The Ogrelord) ( áð ÃÂàáð) ( áð ÃÂàáð) ( áð ÃÂàáð) ( áð ÃÂàáð) by Badfish2
...â ãâ¡ãâ°) ( ãâ¡ãâ° ãâãâ ãâ¡ãâ°) by badfish2
thingiverse
shrek is love. shrek is life. ( ãâ¡ãâ° ãâãâ ãâ¡ãâ°)
blendswap
free
2014 newyear forest
...¹ãâ¹ ãâ¸ãâ½ãâãâãâ¬ãæãâ¼ãâµãâ½ãâ, ãâ²ãâãâµãâ¼ ãâºãâãâ¾ ãâµãâ³ãâ¾ ãâãâ¾ãâ·ãâ´ãâ°ãâ²ãâ°ãâ».
thingiverse
free
Blade-1ã¯ã¼ãªã³ã°ãÂÂã¡ã³ãÂÂãºã« by Hotproceed
...â°ã£ââã£ââ¡ã£ââ³ã£ââã£ââã£ââã£ââ«ã¥â
â¥ã£ââ£ã£ââ¦ã£ââã£ââã£ââã£ââºã£ââ«ã£ââã£ââ¼ã£ââ¿ã£ââ§ã£ââã£ââ
thingiverse
free
N-DO badge by hare081987
...n-do badge by hare081987
thingiverse
ã â¸âã â¸â£ã â¸â²ã â¸âã â¸â±ã â¹âã â¸â¡ã â¹âã â¸âã â¹âã â¸âã â¸âã â¸â¹
Specimens
3ddd
$1
Pinus halepensis
...k is quadrangulated and can be turbosmoothed. the bark has a normal map; for close-ups, it's suggested to use a displacement.
3ddd
$1
Cement_wood_lamp_&_decor
...nanun-detail?itemid=0&show;_cat_id=10
подсвечник muutohttp://www.muuto.com/collection/float_candlestick/
3ddd
$1
Pinus halepensis #2
...e a displacement.
to add variation to your grove, use this model too:http://3dsky.org/3dmodels/show/pinus_halepensis_1
3ddd
$1
RH PETITE GLASS CLOCHE COLLECTION
...l to a petite plant or mineral specimen. made of clear glass and set on ebonized wood stands, they're available in six sizes.
3ddd
$1
Minifix
...arts in the assembly of furniture quickly and practice. a specimen was minutely executed, being a real 3d version of the product.
3d_export
$9
Bamboo table top mesh
...ent.<br>no part-name confusion when importing several models into a scene.<br>no special plugin needed to open scene.
3d_export
$9
Bamboo table
...ent.<br>no part-name confusion when importing several models into a scene.<br>no special plugin needed to open scene.
3d_export
$30
narval
...reaches 3.8-4.5 m (in the old days there were specimens 6 m long), newborns - 1-1.5 m. the weight...
3d_export
$5
colorful flower
... for.<br>• house plants; supplied as 1 x indoor jungle collection of 5 plants<br>polys:160 011<br>verts:161 494
3d_export
$9
Coffee table Bamboo
...<br>- no part-name confusion when importing several models into a scene.<br>- no special plugin needed to open scene.
Biological
3d_ocean
$29
Exterminator
...exterminator 3docean bilogical biological bug bugs character characters chemical coveralls exterminator gas gasmask...
3d_ocean
$29
Man In Protective Hazmat Suit
...man in protective hazmat suit 3docean bilogical biohazard biological character characters chemical coveralls gas gasmask hazmat human man...
cg_studio
$13
Shell barrel3d model
...warehouse storage factory set pack barrel barrels pallet container biological rusty oil 3d model vray gasoline petrol gas shell...
cg_studio
$14
Oil barrel + Pallet3d model
...warehouse storage factory set pack barrel barrels pallet container biological rusty rusted oil 3d model vray gasoline petrol gas...
3d_export
$39
Exterminator 3D Model
...uniform coveralls protection man mask gas respirator insect gasmask biological toxicpoison chemical characters bilogical toxin human exterminator 3d model...
3d_export
$39
Man In Protective Hazmat Suit 3D Model
...low poly game niform coveralls protection gas respirator insect biological man in protective hazmat suit 3d model beatheart 99915...
3d_export
$199
realistic male rigged
...eye model, exposing aesthetic control over each of the biological parts of the eye.<br>- hair shader simulates the reflection...
3d_export
$99
ural 4320
...an ambulance, or for conducting engineering, radiation, chemical and biological reconnaissance. - the model is highly detailed , prepared...
3d_export
$1638
hinged rotary harrow - full documentation
...from the air, which significantly activates (accelerates) the soil biological processes and the accumulation (synthesis) of nitrogen and thereby...
3d_export
$15
spacecraft foton-m
...experiments in orbital flight, the production of materials and biological preparations in the interests of various industries and science,...
Usb
3d_export
$49
Google Chromecast 3D Model
...google chromecast media wi-fi player show films music video usb google chromecast 3d model humster3d 90926...
3d_export
$5
USB Kingston 3D Model
...usb kingston 3d model
3dexport
usb kingston 3d model kostya 49949 3dexport
3d_export
$10
Cartoon Sensei Ninja 3D Model
...sensei ninja 3d model 3dexport ninja sensei warrior cartoon usb cartoon sensei ninja 3d model sap3d 84475...
3d_export
$10
USB Modem Alcatel One Touch X230 3D Model
...usb modem alcatel one touch x230 flash drive computer device memory
usb modem alcatel one touch x230 3d model mkms 53730 3dexport
3d_ocean
$39
Mock Up project c4d
...file locations leaflet mobile phone mock up notebook sticker usb card mock up project c4d in this project mock...
3d_export
$19
Logitech USB Desktop Microphone 3D Model
...stic metall
logitech usb desktop microphone 3d model download .c4d .max .obj .fbx .ma .lwo .3ds .3dm .stl dixiony 106923 3dexport
3d_export
free
Download free USB flash drive 3D Model
...xport
3d model usb flash
usb flash drive 3d model download free .c4d .max .obj .fbx .ma .lwo .3ds .3dm .stl yurex 105333 3dexport
cg_studio
$18
Digital picture frame remote low poly3d model
...tv cable television dvd centre lcd console digital device usb computer monitor screen .obj .max .fbx .3ds - digital...
3ddd
$1
Pioneer network audio player N-70A-K
...player n-70a-k 3ddd pioneer , плеер сетевой аудиоплеер с usb на передней панели, обработкой многобитного сигнала, usb цап и...
cg_studio
$49
Google Chromecast3d model
...chromecast3d model cgstudio chromecast google set-top box tv hdmi usb .max .obj .mb .lwo .fbx .c4d .3ds - google...
Powered
3d_export
$200
Fallout T51B Power Suit 3D Model
...ut t51b power suit 3d model
3dexport
fallout power armor t-51b videogame
fallout t51b power suit 3d model scifipro 38925 3dexport
3d_export
$7
Business Center 3D Model
...er table skyscraper lowpoly game photorealistic collection textures house business
business center 3d model ikaika 74521 3dexport
3d_export
$75
Strong Man 2014 3D Model
...es human male 18 nude anatomy power super superman 3d unity fbx max obj 3ds 3dmax
strong man 2014 3d model zeonman 85537 3dexport
3d_export
$5
Interior Kitchen 3D Model
...r table skyscraper lowpoly game photorealistic collection textures house business
interior kitchen 3d model ikaika 74528 3dexport
3d_export
$15
Classic Steam Train 3D Model
...ocomotive metal old power rail railroad railway retro smoke station steam steel
classic steam train 3d model jronn 65890 3dexport
3d_export
$95
BMW M235i F22 3D Model
... 2-series 2er coupe sport car premium german fast detailed realistic mental ray
bmw m235i f22 3d model fisherman3d 78086 3dexport
3d_export
$110
Power relay station 3D Model
...h poly gun anty-aircraft tower pipes futuristic structure sci fi powerplant plant
power relay station 3d model td3 53026 3dexport
3ddd
$1
RH VICTORIAN HOTEL FLOOR LAMP + DESK LAMP
...our lamp pairs the intricate open metalwork typical of flame-powered lanterns with the modern drama of an internal candelabra....
3d_ocean
$3
Schuko Socket
...nector power power point safety socket schuko socket wall socket
a schuko socket like the ones used in germany. colored old white
3d_ocean
$5
Column Power Lines
...ry iron line metal pole power pylon single steel structure supply tall technology tower voltage wire
column power lines 3d model.
Printed
3ddd
$1
H&M accessories set
...за
decorative set from h&m; home
prints by elisabeth fredriksson
в архиве модель в формате:
-3ds max 2015
-3ds max 2012
-obj
3d_ocean
$5
Butterfly
...in almost all 3d formats it can be 3d printed material included . gold white gold silver colored metal...
3d_ocean
$4
Jewelry
...in almost all 3d formats it can be 3d printed material included gold white gold silver colored metal colored...
3d_ocean
$7
3D Mirror
... decor
3d mirror for decoration, home decor, 3d printing and interior design. this model ready to be machined using 3 axis router
3d_ocean
$4
Flower Jewelry
...flower jewelry
3docean
decoration flower jewelry
just a little 3d model of flower jewelry. ready for 3d printing.
3d_ocean
$4
New Year
...vray it come with these file 3dsmax 2016 2015 2014 2013 maya 2016 obj blender c4d stl for 3d print 3ds fbx keyshot happy new year
3d_ocean
$12
Pumpkin 3D print
...table pumpkin sculpture
5 models ready for 3d print height 4cm archive includes file formats: stl (total and for each model), obj
3d_ocean
$6
Duck
...emblem metal model sculpture soot souvenirs trinkets zbrush
model duck for 3d printing. a great souvenir on the hood of your car.
3d_ocean
$4
New Year
...vray it come with these file 3dsmax 2016 2015 2014 2013 maya 2016 obj blender c4d stl for 3d print 3ds fbx keyshot happy new year
3d_export
$10
Jewelry 3D Model
...tone jewellery precious 3dprint print
jewelry 3d model download .c4d .max .obj .fbx .ma .lwo .3ds .3dm .stl 3dact 110142 3dexport